服务热线
18915418616
AAT Bioquest公司荧光探针Rhod-4™使用说明
苏州蚂蚁淘生物科技有限公司是AATBIO的中国大陆区授权代理商
美国AAT Bioquest Inc.(前身是ABD Bioquest,Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域,包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助*的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品,快速地丰富各个领域的产品。
1)提供反应探针和发光探针,生物素和端粒酶能够应用于标记药物小分子和生物聚合物,如蛋白、核酸以及其他碳水化合物;
2)研究并生产荧光和发光探针用于检测蛋白,核酸和活细胞;
3)不断推出新型的荧光和发光探针用于检测多种酶,特别是检测水解酶和氧化还原酶类;
4)致力于开发用于信号转导研究的试剂;
5)提供生理和神经探针,特别是钙离子指示剂和膜电位探针。
AAT Bioquest公司荧光探针Rhod-4™使用说明
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge
Ex/Em (nm) | 524/551 |
MW | 1015.96 |
Kd (nM) | 451 |
Solvent | DMSO |
Storage | Freeze (<-15 °C) Minimize light exposure |
Instruments | Fluorescence microplate reader, Fluorescence microscope, Flow cytometer |
Category | GPCR Calcium GPCR Assays |
Related | Calcium Channels
|
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 98.429 µL | 492.145 µL | 984.291 µL | 4.921 mL | 9.843 mL |
5 mM | 19.686 µL | 98.429 µL | 196.858 µL | 984.291 µL | 1.969 mL |
10 mM | 9.843 µL | 49.215 µL | 98.429 µL | 492.145 µL | 984.291 µL |
如果你对21120AAT Bioquest公司荧光探针Rhod-4™使用说明感兴趣,想了解更详细的产品信息,填写下表直接与厂家联系: |