AAT Bioquest公司荧光探针Rhod-4™使用说明

AAT Bioquest公司荧光探针Rhod-4™使用说明

苏州蚂蚁淘生物科技有限公司是AATBIO的中国大陆区授权代理商

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括吸收(颜色)、荧光和发光技术。AAT Bioquest的产品帮助*的科学家和生物医药研究者更好的了解生物化学、免疫学、细胞生物学和分子生物学等领域。AAT Bioquest会不断研发新产品，快速地丰富各个领域的产品。

1）提供反应探针和发光探针，生物素和端粒酶能够应用于标记药物小分子和生物聚合物，如蛋白、核酸以及其他碳水化合物；

2）研究并生产荧光和发光探针用于检测蛋白，核酸和活细胞；

3）不断推出新型的荧光和发光探针用于检测多种酶，特别是检测水解酶和氧化还原酶类；

4）致力于开发用于信号转导研究的试剂；

5）提供生理和神经探针，特别是钙离子指示剂和膜电位探针。

AAT Bioquest公司荧光探针Rhod-4™使用说明

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses. Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM. AAT Bioquest offers versatile packing sizes of Quest Rhod-4 to meet your special needs, e.g., 1 mg; 10x50 µg; 20x50 µg; HTS packages with no additional packaging charge

Calculators
Common stock solution preparation 
 

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-4™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.