AAT Bioquest公司荧光探针Fluo-8®使用说明

AAT Bioquest公司荧光探针Fluo-8®使用说明

 苏州蚂蚁淘生物科技有限公司是AATBIO的中国大陆区授权代理商

产品介绍：       

         染料DiI, DiO, DiD 和 DiR是一类亲脂性荧光染料家族，用于标记细胞膜和疏水性组织。这是一类环境敏感型荧光染料，当它们与膜结合或者与亲脂性生物分子(例如蛋白质，虽然在水中其荧光强度很弱)结合时，其荧光强度显著增强。它们具有很高的淬灭系数，偏光依赖性和很短的激发寿命。一旦应用于细胞中，这种染料会在细胞内质膜中逐步扩散，导致在其*浓度条件下，将整个细胞染色。

        它们不同的荧光颜色：DiI（橙色荧光）、DiO（绿色荧光）、DiD（红色荧光）、DiR（深红色荧光），为活细胞多色彩荧光成像分析和流式细胞术提供了一种便捷的工具。DiO和DiI可以分别与标准的FITC和TRITC滤光器一同使用，其中，DiO可以被633 nm He-Ne激光激发，并且具有比DiI更长的激发和发射光波长，为标记细胞和组织的那些本身就具有本底荧光的染料提供了非常的替代品。

        DiR在活体成像或者示踪中非常有用，因为它们所发射的红外光可以高效地穿过细胞和组织，并且在红外光范围内，其本底荧光水平很低。

技术资料：

      1.Ex(nm)：748

      2.Em(nm)：780

      3.分子量：

      4.溶剂：DMSO

AAT Bioquest公司荧光探针Fluo-8®使用说明

 Fluo-8® AM is a novel green fluorescent indicator that utilizes a fluorescein core to monitor Ca2+ concentration and flux in cells. It has several key advantages over earlier generations of indicators like Fluo-3 AM and Fluo-4 AM:

• Enhanced Intensity → 2x brighter than Fluo-4 AM
• Better & Faster Loading → Room temperature instead of 37°C
• More Robust Results → Probe effective in a wide range of temperatures
• Variety of K_d → Many options for calcium binding dissociation constant

Fluo-8® AM Features

Fluo-8® AM is a novel fluorescent green calcium indicator that builds upon the fluorescein core while rectifying the issues that plagued earlier indicators like Fluo-3 AM and Fluo-4 AM. In particular, it has several key advantages:

• Enhanced Intensity - Tests have shown Fluo-8® AM is twice as bright as Fluo-4 AM and up to four times brighter than Fluo-3 AM
• Better Loading - Fluo-8® AM can be loaded at room temperature, unlike the 37°C incubation required by Fluo-4 AM
• Faster Loading - Room temperature loading means no need to warm up and cool down cells; finish loading in as little as 20 minutes
• Robust - Fluo-8® is less temperature dependent than other probes, leading to more consistent results
• Versatile K_d - Fluo-8® AM is available with a variety of Ca2+ binding dissociation constants, see Table 1 for details

The most brilliant achievement of Fluo-8® AM is that it improves upon the shortcomings of Fluo-3 AM and Fluo-4 AM without touching the excitation and emission. Like Fluo-3 AM and Fluo-4 AM, Fluo-8® AM will excite at ~490 nm and emit at ~520 nm. This allows for a seamless transition for those who are familiar with Fluo-3 AM and Fluo-4 AM but wish to upgrade to Fluo-8® AM. 

As with its predecessors, Fluo-8® AM is completely compatible with use in flow cytometry, fluorescence microscopy, fluorescence spectroscopy and the fluorescence microplate platform. To read more about how Fluo-8® AM has revolutionized calcium imaging, or to see additional Fluo-8® product applications, please click here. 

Table 1. Ca2+ Binding Dissociation Constants