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Jackson|二抗009-090-050*
ChromPure是我们来自非免疫动物血清的高纯度蛋白质的商品名。
物理状态:冷冻干燥的固体
存储:
储存和再水化:将冷冻干燥的固体储存在2-8°C。用体积的dH2O再水化,如果不清楚则离心。在使用当天准备工作稀释液。产品在2-8℃下作为未稀释的液体稳定约6周。
再水化后的长期储存:等分并在-70℃或更低温度下冷冻。避免反复冻融。或者,加入等体积的甘油(ACS级或更好),终浓度为50%,并在-20°C下储存为液体。
失效日期:补液之日起一年。如果测试结果对于预期用途是可接受的,则可以延长失效日期。
纯度:基于抗原浓度为20mg / ml的免疫电泳,针对山羊抗人全血清的沉淀模式与针对山羊抗人转铁蛋白的沉淀模式相同。没有检测到针对山羊抗人IgG(H + L)的沉淀素系。
缓冲液:0.01M磷酸钠,0.25M NaCl,pH 7.6
稳定剂:15毫克/毫升牛血清白蛋白(无IgG,无蛋白酶)
防腐剂:0.05%die氮化钠
Jackson|二抗009-090-050*
Amax: 492 Emax: 520nm
FITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is still a widely used fluorophore due to its long history. The major disadvantage of fluorescein is its rapid photobleaching (fading), which can be mitigated by the use of an anti-fading agent in the mounting medium. A better choice for many applications involving FITC is Alexa Fluor® 488 because it is brighter and more photostable.
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