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ThermoFisher | A11029 | Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate |
ThermoFisher | A11034 | Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate |
invitrogen/A11029/A11034二抗*
订货找蚂蚁淘就对了,4000-520-626
Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-11034) in IF
Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-16891). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/ml Rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 488 conjugate (Product # A-11034) was used at a concentration of 4 µg/ml in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Rhodamine Phalloidin (Product # R415, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
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