Jackson|二抗709-005-149*

Jackson|二抗709-005-149*

通过免疫亲和层析从抗血清分离全IgG抗体作为完整分子。 它们具有Fc部分和两个通过二硫键连接在一起的抗原结合Fab部分，因此它们是二价的。 据报道平均分子量约为160kDa。 整个IgG形式的抗体适用于大多数免疫检测程序，并且是成本效益的。

基于免疫电泳和/或ELISA，抗体与全分子人IgG反应。 它还与其他人免疫球蛋白的轻链反应。 未检测到针对非免疫球蛋白血清蛋白的抗体。 已经通过ELISA和/或固相吸附测试抗体以确保与牛，鸡，山羊，豚鼠，叙利亚仓鼠，马，小鼠，兔，大鼠和绵羊血清蛋白的小交叉反应，但它可以交叉 -  与来自其他物种的免疫球蛋白反应。

Jackson|二抗709-005-149*

Physical State: Sterile-filtered liquid 
Storage:

Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. 
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Preservative: None 

Suggested Working Concentration or Dilution Range:

10-20 µg / ml 

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.