Chromotek/Nuclear Actin-Chromobody

Chromotek/Nuclear Actin-Chromobody

蚂蚁淘常见平行进口产品有：

Thermofisher旗下Gibco培养系列、ABI细胞计数仪及PCR仪、Invitrogen分子生物学试剂及Qubit3.0、Ambion的RNA相关试剂、Molecular probes试剂、Pierce蛋白相关试剂盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich试剂；AccuStandard 标准品；Millipore抗体；Glen Research 核酸合成试剂；Bio-Rad电泳仪及PCR仪；Nanocs的PEG聚合物；  Chromotek羊驼抗体；   Polysciences转染试剂；  Echelon-inc 脂类研究试剂盒；   R&D Systems抗体及Elisa试剂盒； Jackson二抗及封闭血清； Biotium 荧光染料；NEB内切酶及NGS相关产品；  Athens Research & Technology人类蛋白及抗血清；  Illumina二代测试试剂盒； Tocris 小分子试剂； TwistDx恒温扩增试剂盒（RPA）； Enzymatics二代测序酶；Novus抗体及Elisa试剂盒；AG scientific潮霉素B、G-418；Plantmedia植物培养系列及抗体；Agilent 2100生物分析仪；Biolog耗材；Biomatik的Elisa试剂盒；Covaris基因打断仪；CST抗体；Dharmacon的siRNA产品。

Chromotek/Nuclear Actin-Chromobody

　　Visualize the actin dynamics in real time in the nucleus with the nuclear Actin-Chromobody®. Upon transformation, your cells express the nuclear Actin-Chromobody® and transport the nuclear Actin-Chromobody into the nucleus. The nuclear cytoskeleton is visualized in real time by binding of the fluorescent Chromobody® to Actin.Actin-Chromobody®

　　Courtesy of Matthias PlessnerNIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody® (TagGFP2, green) and the Lamin-Chromobody® (TagRFP2, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody-probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum.

　　Trace actin dynamics in cell nuclei non-invasively

　　With the nuclear Actin-Chromobody® plasmid you obtain the sequence of the Alpaca antibody to Actin, also termed VHH-Actin or Actin-nanobody, fused to TagGFP (Evrogen) and a nuclear localization sequence (NLS). The transient binding does not influence cell viability or motility and thus offers you the unique possibility to observe the cytoskeleton in your cell nuclei.

　　The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:

　　Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (PubMed)